My nose microbiome throughout the seasons

Does the olfactory microbiome change when the seasons shift?

Springtime for many people brings hay fever, an allergic reaction known to be associated with the microbiome. I fortunately don’t suffer from the condition, but I wondered if maybe I could find something in my nose microbiome that would show a seasonal shift, perhaps something aligned with allergy season. Even if I don’t have symptoms, maybe by finding some of the key microbes involved my data might be useful to others who would like to explore more of the link between their allergies and microbes.

Like every place on the body, your nose has its own unique microbial ecology, as different from other sites as a tropical rainforest is from the artic tundra. Unlike the gut or the mouth, your nose is in constant contact with the external environment, exposed to new microbes that float in day and night with every breath you take.

[Image: Zinniazimminy]

It seems reasonable to expect that we’d see different microbes as the seasons shift. After all, changes in temperature, humidity, and daylight affect the abundance and variety of plants, so of course these shifts will affect microbes. But is there a pattern to the changes?

To find out, I sampled my nose microbiome more than 50 times over a period of three years, carefully tracking the date and microbial species in each sample. In all, I found more than 200 different (genus-level) bacteria from about 350 unique species.

Using a versatile clustering algorithm called non-metric multidimension scaling (NMDS), I calculated the statistical correlations among the hundreds of microbes in a way that let me build a two-dimension chart where similar samples are clustered together, and less-similar samples are further apart. Where there are significant differences among samples, an NMDS chart will show obvious clustering, with similar samples bunched together and separate from other clusters.

My nose microbiome across seasons represented as an NMDS projection. The X-Y axes are generated from the two most statistically-significant elements when comparing all of the samples.

Okay, this chart is kind of a mess. If there were major shifts, we’d see clear clusters. Although we don’t quite see that, it is interesting that the seasons do kinda-sorta hang together, though with much overlap. For example, the (red) winter dots are all on the right side of the chart, with the (blue) summer dots mostly on the left. The (purple) fall dots are, if anything, closer to the winter samples, and (green) spring is closer to summer. Both spring and fall were more in the middle, which you might kinda-sorta expect given that they are generally less extreme than the other two seasons.

Importantly, each of the samples was taken under the same conditions: I swabbed the nostrils for about 30 seconds right after waking up each morning. Since my bedroom is kept at a climate-controlled temperature year-round, these samples wouldn’t show direct results of major seasonal changes, though microbes in my bedroom are presumably affected somewhat by what’s happening outdoors. I also have a number of samples taken in different geographies while traveling — and they were very different — but I removed them before making this chart.

Seasons and Diversity

What about the richness and diversity of microbes throughout the year? The box plot below shows that summer has the widest diversity range (those dots outside the main box are outliers). In this plot, the area in the box represents 50% of the samples, and the line through the middle is the median diversity.

I spend more time indoors in the winter, so perhaps that explains why median diversity is lowest then. Again, it’s interesting that both spring and fall have similar diversities to each other, perhaps because both seasons have similar amounts of indoor-outdoor time and maybe the variety of microbes reflect that.

My nose microbiome diversity across the seasons. All samples were taken in the same geography, first thing after waking in the morning.

Finally, if you’re curious about which microbes are most common in my nose, the following chart shows that the vast majority are Corynebacterium and Moraxella, with a steady small amounts of Dolosigranulum and Propionibacterium. I occasionally have a spike of Staphylococcus, a usually benign and perhaps even beneficial microbe that can turn pathogenic under some circumstances.

Many of the rest of these top-25 microbes are unexplored. Although they represent only a tiny fraction of the life inside the nose, the microbiome is often shaped by a few key species. (In a previous post, I speculated that one of them, Ochrobactrum might have something to do with when I catch colds). Maybe with more exploration of more noses we can uncover some of the ones that might exacerbate or even cause the uncomfortable symptoms of allergies.

Meanwhile, visit https://personalscience.com to see other tests I’ve performed.

Originally published at richardsprague.com on June 7, 2018.